FIGURE 5:

Evaluation of sporulation in cells lacking TAG lipases. (A) Cell strains, as indicated, were grown in SPO for 24 h and stained with CFW and eosin Y or with dityrosine, followed by fluorescence microscopy. Quantification data counted from >150 cells for representative images. Scale bar, 5 μm. (B) Cell strains, as indicated, expressing Htb2-GFP were grown in SPO for 24 h and subjected to fluorescence microscopy. Htb2-GFP is shown as the maximal projection image. Scale bar, 5 μm. (C) Spore viability test for the indicated strains based on tetrad dissection. (D) Lipids isolated from wild-type and tgl3Δ cells were analyzed by LC/MS. Fold change of indicated TAG and FA species relative to the level in wild type during 12 h in SPO is shown. The results are summarized from four independent experimental repeats and shown as mean ± SD. (E) Lipids isolated from wild-type and tgl3Δ cells harvested at the indicated time point were subjected to LC/MS. The intensity of indicated lipid species is quantified and plotted. The results are summarized from four independent experimental repeats and shown as mean ± SD. *p < 0.05; **p < 0.01.