Table 2.
Fluorescence data for the new amidines.
| Compound | λexa,b (nm) | λema,b (nm) | Em. intensityc |
|---|---|---|---|
| DAPI | 343 | 458 | 821 |
| 5a | 322 | 481 | 176 |
| 5b | 351 | 463 | 611 |
| 5c | 341 | 461 | 33 |
| 5d | 351 | 493 | 452 |
| 5e | 348 | 470 | 461 |
| 5f | 339 | 462 | 260 |
| 5g | 342 | 480 | 53 |
| 5h | 358 | 475 | 200 |
| 5i | 322 | 418 | 875 |
| 5j | 348 | 460 | 680 |
| 5k | 370 | 481 | 810 |
| 8 | 312 | 433 | 690 |
a
Wavelengths (λ)are indicated for excitation (ex) and emission (em).
b
Measurements were made with 0.001 M solutions in distilled water.
c
Emission intensities were measured at excitation slit width of 5 and emission slit width of 20.