Table 1.

Collection of Questionnaire and Clinical Data and Biospecimens at Baseline and Follow-up, LEGACY Girls Study, 2011 – 2015

Participants	Baseline	F1a	F2 a	F3 a	F4 a	F5 a	F6 a	F7 a
Mothers	Growth and Development b	Growth and Development	Growth and Development	Growth and Development	Growth and Development	Growth and Development	Growth and Development	Growth and Development
	Behavioral Questionnaire c		Behavioral Questionnaire		Behavioral Questionnaire		Behavioral Questionnaire
	Cancer Family History d
	Update on Cancer Family History: In year 5 of the study
	Early-life Questionnaire e		Update on Home Environment and Medical History f		Update on Home Environment and Medical History		Update on Home Environment and Medical History
		History of Chronic Conditions
		Recent Physical Activity g		Recent Physical Activity		Recent Physical Activity		Recent Physical Activity
		Recent Dietary Intake h		Recent Dietary Intake		Recent Dietary Intake		Recent Dietary Intake
						Nutritioni		Nutrition
		Neighborhood Characteristics j						Neighborhood Characteristics
			Cosmetics				Cosmetics
			Physical and Social Environmentk				Physical and Social Environment
								Medical History and Infections
	Saliva sample l
Daughters ages ≥10 years	Growth and Development b	Growth and Development	Growth and Development	Growth and Development	Growth and Development	Growth and Development	Growth and Development	Growth and Development
		Recent Physical Activity g		Recent Physical Activity		Recent Physical Activity		Recent Physical Activity
		Recent Dietary Intake h		Recent Dietary Intake		Recent Dietary Intake		Recent Dietary Intake
						Nutritioni		Nutrition
		Neighborhood Characteristics j						Neighborhood Characteristics
			Cosmetics				Cosmetics
			Social Environment m				Social Environment
	Behavioral Questionnaire n		Behavioral Questionnaire		Behavioral Questionnaire		Behavioral Questionnaire
	Optical Spectroscopy Measurements: In year 5 of the study
Daughters of all ages	Anthropometryo	Anthropometry	Anthropometry	Anthropometry	Anthropometry	Anthropometry	Anthropometry	Anthropometry
	Clinical Breast Tanner Staging p	Clinical Breast Tanner Staging	Clinical Breast Tanner Staging	Clinical Breast Tanner Staging	Clinical Breast Tanner Staging	Clinical Breast Tanner Staging	Clinical Breast Tanner Staging	Clinical Breast Tanner Staging
	Urine Sample	Urine Sample	Urine Sample	Urine Sample	Urine Sample	Urine Sample	Urine Sample	Urine Sample
	Non-fasting Blood or Saliva Sample	Fasting Blood or Saliva Sample		Fasting Blood Sample		Fasting Blood Sample		Fasting Blood Sample

^aF1: Follow-up 1 at 6 months; F2: Follow-up 2 at 12 months; F3: Follow-up 3 at 18 months; F4: Follow-up 4 at 24 months; F5: Follow-up 5 at 30 months; F6: Follow-up 6 at 36 months; F7: Follow-up 7 at 42 months.

^bGrowth and Development: Signs of puberty (growth spurt, body hair, breast development, skin changes); menses; Tanner stage of breast and pubic hair development; comparative body size (height, weight) currently and at age 6 years; current body shape (drawing).

^cBehavioral Questionnaire: Daughter’s psychosocial adjustment; maternal anxiety, depression, breast cancer-specific distress, perceived stress, knowledge and perceptions of breast cancer risk, breast cancer and genetic testing experiences, family functioning and communication and health behaviors.

^dCancer Family History: Cancer in daughter’s 1^st, 2^nd, and 3^rd degree relatives; familial relationship, type of cancer diagnosed, age at diagnosis.

^eEarly-life Questionnaire: Daughter’s demographic background; parents’ background (demographics, income, occupation, country of birth, migration to US/Canada, language); parents’ height; parents’ physical activity; grandparents’ country of birth; home environment (primary residence, language spoken, household composition); siblings (Age, gender, age at menarche for sisters); pregnancy and birth outcomes (weight gain, physical activity, smoking, alcohol consumption, use of prenatal vitamins, duration of pregnancy, medical conditions, birth weight and length, breast-feeding); medical history (medication use, medical conditions, chronic conditions); daughter’s physical activity level at ages 3–5 years; lifetime history of organized sports and exercise activities.

^fUpdate on Home Environment and Medical History: Home environment; parents’ occupation; medical history.

^gRecent Physical Activity: Physical education (PE) at school, transportation to school, organized sports and activities in past year, organized sports and activities in past week, other physical activities in past week, sedentary activities and sleeping on prior day.

^hRecent Dietary Intake: Food Frequency Questionnaire on intake in past week (frequency, portion size).

ⁱNutrition: Intake of organic food, soy food, fast-food and restaurant food, family meals, special diet.

^jNeighborhood Characteristics: Type of housing, walkability, safety, availability of healthy foods.

^kPhysical and Social Environment: Chemical exposure in home and community environment; attitudes about daily life; place in society.

^lSaliva: Collected unless DNA is already available in the Breast Cancer Family Registry biorepositories.

^mSocial Environment: Place in society; future hopes.

ⁿBehavioral Questionnaire: Daughter’s psychosocial adjustment, breast cancer-specific distress, perceived stress, knowledge and perceptions of breast cancer risk, breast cancer experiences, family functioning and communication, health and risk behaviors.

^oAnthropometry: Standing height, weight, percent body fat, waist and hip circumference, foot size.

^pClinical Breast Tanner Staging: Performed at New York and Utah study sites only.