Table 3.

Effects of the Experimental Diets on Hepatic Histology at 30 Weeks

Diet (N = 8 animals for each)	Control	HC	HF	HFHC
Steatosis (0–3)*	0	2	2	3
Lobular inflammation (0–3)†	0	1	1	2
Fibrosis (0–4)‡	0	0	0	1a
Hepatic Sirius Red staining, %	0.50 ± 0.21	0.88 ± 0.63	0.65 ± 0.52	1.54 ± 1.11

Median values are reported for steatosis, inflammation, and fibrosis and mean ± SD for percent Sirius Red staining.

^*Steatosis is graded as: <5% (0); 5%–33% (1); 34%–66% (2); and >66% (3).

^†Lobular Inflammation combines mononuclear, fat granulomas, and polymorphonuclear leucocytes and is graded as: none (0); <2 per ×200 magnification (1); 2–4 per ×200 magnification (2); and >4 per ×2,000 magnification (3).

^‡Fibrosis is staged as: none (0); perisinusoidal (1a); periportal (1b); periportal and perisinusoidal (2); bridging fibrosis (3); and cirrhosis (4).