Table 3. Validated of distinct and known variants in mismatch repair, tumor suppressors and oncogenes.

	Loci	Ref	Var	Gene	Variant type	Novel	Frequency of Mutation
Mismatch Repair
	80149981	A	G	MSH3	nonsynonymous SNV	0	0.60
	80168937	G	A	MSH3	nonsynonymous SNV	0	0.51
	79950724	G	C	MSH3	nonsynonymous SNV	0	0.02
	48023115	T	C	MSH6	synonymous SNV	0	0.14
	48030588	C	T	MSH6	stopgain	0	0.06
Tumor Suppressor
	63410110	T	C	AMER1	synonymous SNV	0	0.08
	63412690	A	C	AMER1	nonsynonymous SNV	0	0.06
	63411276	G	A	AMER1	stopgain	0	0.06
	63411684	G	T	AMER1	nonsynonymous SNV	1	0.06
	112162854	T	C	APC	synonymous SNV	0	0.52
	112164561	G	A	APC	synonymous SNV	0	0.49
	112176325	G	A	APC	synonymous SNV	0	0.49
	112176559	T	G	APC	synonymous SNV	0	0.54
	112177171	G	A	APC	synonymous SNV	0	0.59
	112176756	T	A	APC	nonsynonymous SNV	0	0.57
	112175770	G	A	APC	synonymous SNV	0	0.49
	112164586	C	T	APC	stopgain	0	0.06
	7579472	G	C	TP53	nonsynonymous SNV	0	0.40
	48604664	C	T	SMAD4	nonsynonymous SNV	1	0.08
Oncogenes
	70118935	C	T	SOX9	synonymous SNV	0	0.13
	114912121	G	A	TCF7L2	synonymous SNV	0	0.08
	114910829	A	G	TCF7L2	synonymous SNV	0	0.05
	30732970	G	A	TGFBR2	nonsynonymous SNV	0	0.06
	30713842	C	T	TGFBR2	synonymous SNV	0	0.02
	178916890	C	T	PIK3CA	nonsynonymous SNV	0	0.06
	178952085	A	G	PIK3CA	nonsynonymous SNV	0	0.06
	25368462	C	T	KRAS	synonymous SNV	0	0.67