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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Nat Microbiol. 2017 Mar 6;2:17027. doi: 10.1038/nmicrobiol.2017.27

Figure 3. WarA impact LPS modal distribution.

Figure 3

a, Model of the heteropolymeric OSA biosynthesis via the Wzy/Wzx-dependent pathway. Individual repeating units are assembled in the cytoplasm and transported via a flippase across the inner membrane to the periplasmic space. Here they are assembled into the O antigen polymer by the Wzy polymerase. Wzz1 and Wzz2 regulate chain length. b, Model of CPA biosynthesis via the ABC transporter pathway. Like LPS biosynthesis in E.coli O9a, synthesis and assembly occur in the cytoplasm via the glycosyltransferases WbpX, WbpY and WbpZ. The assembled O antigen component is then transported via the Wzm/Wzt ABC transporter across the inner membrane. c, Western blot analysis using OSA specific antibody MF83-1 on PAK wild type with an empty vector (+ Ev), PAK wild type overexpressing warA (+ warAOe), a sadC and a warA mutant. d, Western blot analysis using CPA specific antibody (N1F10) on PAK wild type with an empty vector (+ Ev), PAK wild type overexpressing warA (+ warAOe), a sadC and a warA mutant. Representative images of at least two biological replicates shown.