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. 2017 Feb;17(1):12–16. doi: 10.17305/bjbms.2016.1553

FIGURE 2.

FIGURE 2

The analysis of (A) phospho-Akt1 (p-Akt1) and (B) cytochrome c protein expressions in four groups of neuronal cells, using Western blot analysis. Oxygen-glucose deprivation (OGD) significantly down-regulated the level of p-Akt-1 and up-regulated the level of cytochrome c in OGD group. Nevertheless, DL-2-amino-3-phosphonopropionic acid (DL-AP3) significantly recovered the decreased levels of p-Akt1 and the increase of cytochrome c in OGD + DL-AP3 group. DL-AP3: DL-2-amino-3-phosphonopropionic acid; OGD: Oxygen-glucose deprivation; Control: Control group; DL-AP3: DL-AP3 group was treated with 10µM of DL-AP3 for 6 hours; OGD: OGD group in which neurons were cultured under OGD conditions for 12 hours; OGD + DL-AP3: OGD + DL-AP3 group, in which OGD model was first established, and then, the cells were treated with 10 µM of DL-AP3 for 6 hours; ns: No significance; *p < 0.05; ***p < 0.001.