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. 2017 Feb 1;144(3):487–498. doi: 10.1242/dev.143776

Fig. 3.

Fig. 3.

Validation of Rac FRET probe and Abl/Rac epistasis. Abl-responsive or Rac-responsive CFP-YFP unimolecular FRET probes were expressed in Drosophila under the control of GMR-GAL4 and ratiometric FRET was imaged. (A) Single cultured photoreceptors of the indicated genotypes expressing the Raichu-Rac FRET probe. Top panel of each pair shows probe distribution (YFP) and bottom panel shows pseudocolored FRET channel with color scale. Scale bar: 10 μm. (B) Mean Rac FRET ratio for each genetic background. (C) Mean Abl FRET ratio in genotypes that alter trio or Rac1 activity. (D) Mean Rac FRET ratio in genotypes that alter Abl activity, or in WT with and without 30 min treatment with STI-571. (E) Single cultured photoreceptors expressing the Raichu-Rac FRET bioprobe. (F) Timecourse of Raichu-Rac FRET signal in photoreceptors expressing UAS-Abl upon treatment with the Abl inhibitor STI-571 and washout. Signals are normalized to FRET ratio at start of experiment. Error bars indicate s.e.m. *P<0.05, **P<0.01, ***P<0.001.