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. 2016 Sep 10;7(41):66386–66397. doi: 10.18632/oncotarget.11964

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Copyright: © 2016 Noguera et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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a. PTEN mRNA levels steadily increase in lysates from NB4 cells treated with ATRA, compared to DMSO. **: p<0.005 by unpaired t test. b. PTEN protein levels steadily increase in a time- and dose dependent manner in lysates from NB4 cells treated with ATRA, compared to DMSO. **: p<0.005 by unpaired t test. c. Induction of PTEN mRNA by ATRA in primary APL samples. d. PTEN protein is induced by ATRA in primary APL samples. e. Upon ATRA treatment, there is a parallel increase of PU.1 and PTEN protein expression. f. PTEN and PU.1 protein levels steadily increase upon ATRA treatment in lysates from OCI-AML2 and HL60 cells. g. ATO induces PTEN mRNA expression in lysates from NB4 cells. h. PML/RARA protein is induced at 2 hours in PR9 cells treated with ZnSO4 and results in a decrease of PTEN mRNA. The position of the PML/RARA protein is indicated by the arrowhead. i. PML/RARA reduces PTEN half-life. HEK293T cells transfected with GFP-PTEN and PSG5-PML/RARA constructs or GFP-PTEN and (PSG5) control plasmid were treated with cycloheximide (CHX) (100μg/ml) over 24 hours. PML/RARA enhanced PTEN degradation at 24 hours. **: p<0.005 by unpaired t test. j. Proteasome inhibition rescues PTEN expression. The addition of the proteasome inhibitor MG132 to HEK293T cells transfected with the GFP-PTEN and PSG5-PML/RARA constructs or with GFP-PTEN and PSG5 control plasmid rescues PTEN from PML/RARA-induced degradation.

a. PTEN mRNA levels steadily increase in lysates from NB4 cells treated with ATRA, compared to DMSO. **: p<0.005 by unpaired t test. b. PTEN protein levels steadily increase in a time- and dose dependent manner in lysates from NB4 cells treated with ATRA, compared to DMSO. **: p<0.005 by unpaired t test. c. Induction of PTEN mRNA by ATRA in primary APL samples. d. PTEN protein is induced by ATRA in primary APL samples. e. Upon ATRA treatment, there is a parallel increase of PU.1 and PTEN protein expression. f. PTEN and PU.1 protein levels steadily increase upon ATRA treatment in lysates from OCI-AML2 and HL60 cells. g. ATO induces PTEN mRNA expression in lysates from NB4 cells. h. PML/RARA protein is induced at 2 hours in PR9 cells treated with ZnSO4 and results in a decrease of PTEN mRNA. The position of the PML/RARA protein is indicated by the arrowhead. i. PML/RARA reduces PTEN half-life. HEK293T cells transfected with GFP-PTEN and PSG5-PML/RARA constructs or GFP-PTEN and (PSG5) control plasmid were treated with cycloheximide (CHX) (100μg/ml) over 24 hours. PML/RARA enhanced PTEN degradation at 24 hours. **: p<0.005 by unpaired t test. j. Proteasome inhibition rescues PTEN expression. The addition of the proteasome inhibitor MG132 to HEK293T cells transfected with the GFP-PTEN and PSG5-PML/RARA constructs or with GFP-PTEN and PSG5 control plasmid rescues PTEN from PML/RARA-induced degradation.