Figure 4. Spleen size and immunophenotypic analysis of MLL-AF9/NRas^G12D induced leukemic recipients.

A. Spleen length in control Smyd2^+/+ and Smyd2^−/− mice (n = 2 each) and recipients with leukemia derived from either Smyd2^+/+ or Smyd2^−/− HSPCs (n = 20 and n = 16, respectively). No significant difference was observed between the two leukemic genotypes (p=0.4; Student's t-test). B. Representative immunophenotypic analysis of Venus-positive peripheral blood cells from Smyd2^+/+ and Smyd2^−/− leukemic recipients: detection of Venus positivity, Forward scatter (FSC), GR1 and CD3 staining were assessed by flow-cytometry, as indicated.