Table 2. Breeding strategy for the derivation of Smyd2/3 double knockout mice and observed frequencies of each genotype.
| i) Breeding: Smyd2+/−; Smyd3+/− with Smyd2+/−; Smyd3−/− mice | ||
|---|---|---|
| Genotype of pups (Smyd2; Smyd3) | Observed Frequency (nr.) | Expected Frequency (nr.) |
| +/+ +/− |
0% (0) | 12.5% (2) |
| +/+ −/− |
31.25% (5) | 12.5% (2) |
| +/− +/− |
25% (4) | 25% (4) |
| +/− −/− |
12.5% (2) | 25% (4) |
| −/− +/− |
25% (4) | 12.5% (2) |
| −/− −/− |
6.25% (1) | 12.5% (2) |
| Total | 100% (16) | |
| ii) Breeding: Smyd2+/−; Smyd3−/− with Smyd2−/−; Smyd3+/− mice | ||
|---|---|---|
| Genotype of pups (Smyd2/Smyd3) | Observed Frequency (nr.) | Expected Frequency (nr.) |
| +/− +/− |
34.78 % (24) | 25% (17-18) |
| +/− −/− |
20.28 % (14) | 25% (17-18) |
| −/− +/− |
26.08% (18) | 25% (17-18) |
| −/− −/− |
18.84% (13) | 25% (17-18) |
| Total | 100% (69) | |
| iii) Breeding: Smyd2−/−; Smyd3−/+ with Smyd2−/−; Smyd3−/+ mice | ||
|---|---|---|
| Genotype of pups (Smyd2/Smyd3) | Observed Frequency (nr.) | Expected Frequency (nr.) |
| −/− +/+ |
15.625% (5) | 25% (8) |
| −/− +/− |
56.25% (18) | 50% (16) |
| −/− −/− |
28.125% (9) | 25% (8) |
| Total | 100% (32) | |