Figure 10.

Western blot analysis of SW1990 cells treated with 50 μM 1,3,4-O-Bu₃ManNAc, 30 nM of erlotinib, or both compounds in combination showed (A) EGFR levels that are not affected by the analog alone are decreased by erlotinib. (B) EGFR phosphorylation is inhibited by both compounds individually as well as in combination. (C) and (D) p-AKT and p-ERK1/2 levels, respectively, are not significantly affected by any of the treatment conditions. (E) p-STAT3 levels are inhibited by the analog but are not affected by erlotinib; however the combination of the two compounds leads to significantly lower p-STAT3 levels when compared to the analog alone. At least 3 biological replicates were carried out for each experiment with data expressed as mean ± standard error mean (SEM). * indicates a p value of < 0.05.