Figure 4. IFNγ induces IDO1 in RCC and normal renal proximal tubular epithelial cells, and levels are unaffected by IDO inhibition.

A. Human RCC cells (A-498; ACHN; 50 ng/ml hIFNγ) or RENCA cells (100 ng/ml mIFNγ) were incubated with IFNγ for the times indicated, followed by immunolotting for IDO1. B. Human RCC cells, RENCA cells, or normal human RPTEC were incubated with IFNγ in the presence or absence of MTH-trp for 72 h followed by immunoblotting for IDO1. Data shown are representative of at least two independent experiments.