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. 2016 Aug 27;7(41):66540–66557. doi: 10.18632/oncotarget.11658

Figure 5. IFNα induces IDO1 in human RCC and RENCA cells.

Figure 5

The indicated RCC cells were incubated with IFNα or IFNγ for the times indicated and immunoblotted for IDO1 and vinculin or β-actin. A. A498 cells stimulated with either IFNγ for 72h or IFNα for 24-72h. Chemiluminescent exposure times were the same for all samples. B. A498 cells stimulated with IFNα for 24-72h from A. after a longer exposure. C. Caki-1 cells stimulated with IFNα for 24-72h. D. Caki-1 cells stimulated with either IFNγ for 72h or IFNα for 24h. E. RENCA cells stimulated with IFNα for 24-72h. F. RENCA cells stimulated with IFNγ from Figure 4A and RENCA cells stimulated with IFNα from Figure 5E exposed for the same length of time. Data shown are representative of two independent experiments.