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. 2016 Aug 27;7(41):66540–66557. doi: 10.18632/oncotarget.11658

Figure 6. The IFNγ-induced increase in tryptophan catabolism is inhibited by MTH-trp.

Figure 6

Cells were treated as indicated for 72 h and conditioned media subjected to HPLC to measure tryptophan (TRP) and kynurenine (KYN) levels using authentic standards. A. Dose curve for IFNγ (0-50 ng/ml) added to 786-O cells (n = 2) alone, or B. IFNγ (50 ng/ml) in combination with MTH-trp (0-100 μM). Media was collected from C. ACHN, D. A-498, E. RENCA and F. RPTEC cells (n = 3) treated with either human IFNγ (50 ng/ml) or mouse IFNγ (100 ng/ml) with or without MTH-trp (100 μM). Control cells (Con) were treated with 0.1% DMSO only. Results are representative of two independent experiments and expressed as concentration in media per 1 × 106 (1M) cells. **P < 0.01; *P < 0.05. BD: below limits of detection (5 μM).