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. 2016 Aug 20;7(41):66647–66659. doi: 10.18632/oncotarget.11336

Figure 2. The ARC level in cochlear HCs decreased after neomycin injury.

Figure 2

(A) Immunofluorescence staining showed that ARC expression decreased during neomycin treatment. The cochlear sensory epithelium was dissected from P7 mice and cultured with 0.5 mM neomycin for 8 h or 24 h. After neomycin was removed, the tissues were cultured in serum-free medium for an additional 24 h. (B and C) The immunoblotting confirmed that the expression of ARC decreased during neomycin treatment. The protein loading was illustrated using an anti β-actin antibody. (D) Cochlear HC counts revealed a gradual loss of HCs, and this was much slower than the rate of reduction of the ARC protein level. Data are shown as mean ± S.D.*p < 0.05. Scale bars = 20 μm.