Skip to main content
. 2016 Aug 20;7(41):66647–66659. doi: 10.18632/oncotarget.11336

Figure 4. The percentages of both dead and apoptotic HEI-OC-1 cells increased after neomycin injury when ARC was inhibited by siRNA.

Figure 4

(A–C) Flow cytometry analysis showed that the percentages of both dead and apoptotic cells were significantly increased with 10 mM neomycin treatment for 24 h compared to the undamaged controls. The ARC-siRNA-transfected cells had significantly higher percentages of dead and apoptotic cells than the negative-siRNA-transfected controls after 10 mM neomycin treatment for 24 h. (D and E) TUNEL staining showed that the percentage of TUNEL-positive apoptotic cells increased in the ARC-siRNA-transfected groups compared with the negative-siRNA-transfected controls. Data are shown as mean ± S.D. *p < 0.05. Scale bars = 20 μm.