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. 2016 Aug 20;7(41):66647–66659. doi: 10.18632/oncotarget.11336

Figure 7. ARC inhibition with siRNA elevated the ROS and pro-oxidant factor levels and decreased antioxidant factor levels.

Figure 7

(A–C) The immunofluorescence and flow cytometry analysis showed that Mito-SOX intensity was increased after 10 mM neomycin treatment for 24 h compared with the undamaged controls, and Mito-SOX intensity in the ARC-siRNA-transfected groups was significantly increased compared with the negative-siRNA controls. (D) RT-qPCR showed that the expression of the pro-oxidant factor Alox15 and the antioxidant factors Gsr and Sod1 were increased after 10 mM neomycin treatment for 24 h compared with the undamaged controls. (E) RT-qPCR showed that the expression of the antioxidant factors Gsr, Sod1, Nqo1, and Glrx were significantly decreased, while the expression of the pro-oxidant factor Alox15 was significantly increased in the ARC-siRNA-transfected groups compared with the negative siRNA controls. Data are shown as mean ± S.D. *P < 0.05. Scale bars = 20 μm.