Figure 2. Total ratio of periodontal pathogens in H. pylori-positive and -negative subgingival plaque samples.

Real-time PCR detection of periodontal pathogens including Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nuclearum, Treponema denticola, Aggregatibacter actinomycetemcomitans and Tannerella forsythus. The proportion of target bacteria was calculated by comparing the circulation threshold of target bacteria to that of the universal primer. The total ratio of periodontal pathogens was added by calculating the mean of each bacterium.