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. 2016 Aug 20;7(41):66700–66712. doi: 10.18632/oncotarget.11449

Figure 3. H. pylori up-regulated expression of Wnt5a in mRNA and protein levels.

Figure 3

(A) H. pylori was used to infect THP-1 cells at various times at a ratio of 100:1. Real-time PCR was used to detect the expression of Wnt5a mRNA compared to that of GAPDH. (B) H. pylori was used to infect the THP-1 cells for 8 hrs at ratios of 10:1, 20:1, 50:1, 100:1, 150:1 and 200:1. (C) THP-1 cells were stimulated by cagA+ H. pylori and cagA- H. pylori for 8 hrs at ratios of 0, 100:1 and 200:1. Cells were extracted, and total protein was obtained. Western blot was used to detect protein by antibody against Wnt5a, and β-actin was used as the protein control. Real-time PCR was used to detect the expression of Wnt5a mRNA. Data were expressed as mean ± SD from 3 experiments. *p < 0.05; **p < 0.01 by one-way ANOVA.