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. 2016 Aug 20;7(41):66700–66712. doi: 10.18632/oncotarget.11449

Figure 4. The expression of IL-8 in mRNA and protein levels after stimulation of cagA+ H. pylori and cagA− H. pylori.

Figure 4

(A) H. pylori was used to infect THP-1 cells for various times at a ratio of 100:1. Real-time PCR was used to detect the expression of IL-8 mRNA. (B) H. pylori was used to infect THP-1 cells for 8 hrs at ratios of 0, 10:1, 20:1, 50:1, 100:1, 150:1 and 200:1. Real-time PCR was used to detect the expression of IL-8 mRNA. (C) H. pylori was used to infect the THP-1 cells for various times at a ratio of 100:1. ELISA was used to detect the secretion of IL-8. (D) H. pylori was used to infect THP-1 cells for 8 hrs at ratios of 0, 10:1, 20:1, 50:1, 100:1, 150:1 and 200:1. ELISA was used to detect the secretion of IL-8. Data were expressed as mean ± SD from 3 experiments. (E) cagA+ H. pylori and cagA− H. pylori were used to infect the THP-1 cells for 4 hrs and 8 hrs at a ratio of 100:1. (F) cagA+ H. pylori and cagA− H. pylori were used to infect the THP-1 cells for 8 hrs at ratios of 0, 100:1 and 200:1. Data were expressed as mean ± SD from 3 experiments. *p < 0.05; **p < 0.01 by one-way ANOVA.