Table 4. Primers for real-time PCR to detect the expression of target genes.

Primers	Product size (bp)	Target gene	Annealing temperature (°C)
Wnt5a
5′-TCCTCCGTGTTGTGATGTGA-3′	225	Wnt5a mRNA	60
5′-GATACGCTGCAACACCTCTG-3′
GAPDH
5′-ACCACAGTCCATGCCATCAC-3′	226	GAPDH mRNA	60
5′-ACCACCCTGTTGCTGTA-3′
IL-8
5′-TCAGAGACAGCAGAGCACAC-3′	159	IL-8 mRNA	60
5′-GGCAAAACTGCACCTTCACA-3′