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. 2016 Aug 26;7(41):66809–66821. doi: 10.18632/oncotarget.11631

Figure 4. The sensitivity of JQ1 is dependent on the status of PTEN in endometrial cancer cells.

Figure 4

(A) Knockdown PTEN expression by a specific shRNA-PTEN significantly reduced the expression of PTEN in the two endometrial cancer cell lines (B and C) Hec-1a and KLE cells with PTEN loss became less sensitive to JQ1 (IC50 = 420 nM for Hec-1a and 3350 nM for KLE). (D and E) PTEN loss eliminated the G1 phase arrest caused by JQ1 in Hec-1a and KLE cells after 24 hours of treatment (p = 0.025 and p = 0.019 respectively). Figure D and E represent one of three independent experiments. (F) The protein level of PTEN was notably elevated in AN3CA and Ishikawa cell lines with stably transfection of pcDNA3-PTEN plasmids. (G and H) the AN3CA and Ishikawa cells transfected by pcDNA3-PTEN significantly increased the sensitivity of JQ1 compared to their parental cells after 72 hours of treatment (p = 0.006 and p = 0.013 respectively). (I and J) JQ1 induced significant G1 phase arrest in AN3CA and Ishikawa cells with PTEN overexpression after 24 hours of treatment (p = 0.012 and p = 0.02 respectively). Figure (I and J) represent one of three independent experiments.