Figure 6. TAMs from ARF−/− xenografts overexpressed M2 markers and modulate angiogenic genes.

mRNA expression of (A) M2 markers (Ym-1, MRC1/CD206), (B) (TIMP-3) and (C) angiogenic factors (MMP-2, MMP-9 and VEGF) were evaluated by quantitative PCR in TAMs isolated from tumor xenografts from WT and ARF−/− mice. In all cases, mRNA induction levels were normalized to 36B4 mRNA expression. Data are means of each group ± S.D. (n = 4). *P < 0.05, and **P < 0.01 with respect to WT mice.