Figure 5. Variation of RNA integrity in primary tumors and metastases in a metastatic cancer case A129.

(A) One primary pancreatic tumor was bread-loafed as indicated by primary tumor section S2 to S6. Each slice was further cut into equally sized small pieces (approximately 1 × 1 cm) and numbered accordingly to generate sample IDs. Samples with high tumor cellularity were used for RNA extraction and determination of RNA integrity. Samples indicated by an asterisk (*) were excluded due to low tumor cellularity or necrosis. (B) Each piece of tissue was trisected and RNA extracted (E1,E2,E3). The color code for RIN value is the same as in Figure 1.