Table 2. Primer sequence for quantitative real-time polymerase chain reaction (qTR-PCR) assay.
Target gene | Primer sequence |
---|---|
miR-21 | F: 5′-ACACTCCAGCTGGGTAGCTTAT-3′ |
R: 5′-TGGTGTCGTGGAGTCG-3′ | |
U6 | F: 5′-CTGCTTCGGCAGCACA-3′ |
R: 5′-AACGCTTCACGAATTTGCGT-3′ | |
E-cadherin | F: 5′- CAACGACCCAACCCAAGAA-3′ |
R: 5′- CCGAAGAAACAGCAAGAGCA-3′ | |
N-cadherin | F: 5′-TTGGTTTGGGGAGGGAGA-3′ |
R: 5′-CTGGGGTCAGAGGTGTATCATTT-3′ | |
β-catenin | F: 5′-TGCCAAGTGGGTGGTATAGAGG-3′ |
R: 5′-CGCTGGGTATCCTGATGT-3′ | |
Vimentin | F: 5′-GGCTCAGATTCAGGAACAGC-3′ |
R: 5′-GCTTCAACGGCAAAGTTCTC-3′ | |
Slug | F: 5′-CAAGGACACATTAGAACTCACAC-3′ |
R: 5′-CTACACAGCAGCCAGATTC-3′ | |
PTEN | F: 5′-CCAAGCTTATGACAGCCATCATC-3′ |
R: 5′-CGCGGATCCTCAGACTTTTGTAA-3′ | |
β-actin | F: 5′-GAATCAATGCAAGTTCGGTTCC-3′ |
R: 5′-TCATCTCCGCTATTAGCTCCG-3′ |
Note: F, forward; R, reverse.