Figure 7.
A. Immunoblotting analysis of proteins (γH2AX, phos-CHK1, phos-CHK2 and phos-p53) related to the DNA damage pathway. β-Actin was assessed as a loading control. B. The cell cycle distribution histograms of MCF-7 cells treated with [Cu(L)(Ind)NO3] and HSA-[Cu(L)] at the same concentration of 1.4 μM for 24 h. C. The expression levels of CDK1 and cyclin B1 in MCF-7 cells induced by [Cu(L)(Ind)NO3] and HSA-[Cu(L)] at the same concentration (1.4 μM), determined by Western blot analysis. Cells untreated are used as a control, and β-actin is the loading control. Each experiment group is repeated three times.