Table 3. Identification of lipid species from human MCF-10A mammary epithelial cells and MCF-7 and MDA-MB-231 human breast cancer cells by nano-electrospray mass spectrometry, along with p values and relative changes in identified peak intensities.
| Ion mode | Ion species | m/z | Proposed composition | MCF-7/MCF-10A | MDA-MB-231/ MCF-10A | MDA-MB-231/MCF-7 | |||
|---|---|---|---|---|---|---|---|---|---|
| p | Fold change | p | Fold change | p | Fold change | ||||
| PC | |||||||||
| (+) | [M + H]+ | 758 | C16:1/C18:1 | 0.000 | 1.99 (↑) | 0.000 | 2.47 (↑) | 0.136 | 1.24 (ns) |
| (+) | [M + H]+ | 784 | C18:1/C18:2 | 0.000 | 2.40 (↑) | 0.004 | 4.10 (↑) | 0.063 | 1.71 (ns) |
| (+) | [M + H]+ | 786 | C18:1/C18:1 | 0.000 | 2.54 (↑) | 0.002 | 4.07 (↑) | 0.064 | 1.60 (ns) |
| (+) | [M + H]+ | 798 | C18:3/C19:0 | 0.287 | 1.43 (ns) | 0.636 | 1.16 (ns) | 0.240 | 0.81 (ns) |
| (+) | [M + H]+ | 808 | C18:2/C20:3 | 0.000 | 3.19 (↑) | 0.005 | 4.48 (↑) | 0.223 | 1.40 (ns) |
| (+) | [M + H]+ | 810 | C18:0/C20:4 | 0.004 | 1.59 (↑) | 0.000 | 4.48 (↑) | 0.000 | 2.81 (↑) |
| (+) | [M + H]+ | 814 | C18:1/C20:1 | 0.000 | 1.87 (↑) | 0.829 | 1.02 (ns) | 0.000 | 0.55 (↓) |
| (+) | [M + H]+ | 816 | C18:1/C20:0 | 0.001 | 1.57 (↑) | 0.000 | 1.83 (↑) | 0.145 | 1.16 (ns) |
| (+) | [M + H]+ | 818 | C18:0/C20:0 | 0.192 | 1.51 (ns) | 0.017 | 2.28 (↑) | 0.134 | 1.51 (ns) |
| (+) | [M + H]+ | 834 | C18:1/C22:5 | 0.000 | 1.57 (↑) | 0.000 | 1.52 (↑) | 0.634 | 0.97 (ns) |
| plasmenyl-PC | |||||||||
| (+) | [M + H]+ | 800 | C20:0/C18:1 | 0.000 | 1.93 (↑) | 0.859 | 0.95 (ns) | 0.004 | 0.49 (↓) |
| plasmenyl-PE | |||||||||
| (+) | [M + H]+ | 704 | C18:0/C16:0 | 0.108 | 1.49 (ns) | 0.028 | 1.53 (↑) | 0.886 | 1.02 (ns) |
| SM | |||||||||
| (+) | [M]+ | 703 | D18:1/16:0 | 0.750 | 1.09 (ns) | 0.094 | 1.50 (ns) | 0.157 | 1.37 (ns) |
| PE | |||||||||
| (−) | [M − H]− | 742 | C18:1/C18:1 | 0.000 | 1.62 (↑) | 0.000 | 0.47 (↓) | 0.000 | 0.29 (↓) |
| (−) | [M − H]− | 746 | C17:0/C19:0 | 0.000 | 2.62 (↑) | 0.047 | 1.56 (↑) | 0.002 | 0.60 (↓) |
| (−) | [M − H]− | 764 | C18:1/C20:4 | 0.000 | 2.68 (↑) | 0.035 | 1.23 (↑) | 0.000 | 0.46 (↓) |
| (−) | [M − H]− | 792 | C18:1/C22:4 | 0.000 | 1.67 (↑) | 0.002 | 1.55 (↑) | 0.350 | 0.93 (ns) |
| plasmenyl-PE | |||||||||
| (−) | [M − H]− | 750 | C18:0/C20:4 | 0.000 | 0.36 (↓) | 0.000 | 2.87 (↑) | 0.000 | 7.90 (↑) |
| PS | |||||||||
| (−) | [M − H]− | 758 | C16:1/C18:1 | 0.000 | 1.98 (↑) | 0.000 | 3.32 (↑) | 0.001 | 1.67 (↑) |
| (−) | [M − H]− | 760 | C16:0/C18:1 | 0.000 | 1.48 (↑) | 0.000 | 2.44 (↑) | 0.000 | 1.65 (↑) |
| (−) | [M − H]− | 762 | C16:0/C18:0 | 0.006 | 1.22 (↑) | 0.000 | 2.06 (↑) | 0.000 | 1.70 (↑) |
| (−) | [M − H]− | 774 | C17:0/C18:1 | 0.000 | 2.46 (↑) | 0.025 | 1.21 (↑) | 0.000 | 0.49 (↓) |
| (−) | [M − H]− | 784 | C18:1/C18:2 | 0.000 | 0.60 (↓) | 0.000 | 3.29 (↑) | 0.000 | 5.45 (↑) |
| (−) | [M − H]− | 786 | C18:0/C18:2 | 0.796 | 0.99 (ns) | 0.000 | 2.12 (↑) | 0.000 | 2.15 (↑) |
| (−) | [M − H]− | 788 | C18:0/C18:1 | 0.007 | 0.86 (↓) | 0.000 | 2.04 (↑) | 0.000 | 2.37 (↑) |
| (−) | [M − H]− | 790 | C18:0/C18:0 | 0.000 | 2.01 (↑) | 0.000 | 2.11 (↑) | 0.241 | 1.05 (ns) |
| (−) | [M − H]− | 810 | C18:0/C20:4 | 0.468 | 1.07 (ns) | 0.000 | 4.43 (↑) | 0.000 | 4.15 (↑) |
| (−) | [M − H]− | 816 | C18:1/C20:0 | 0.000 | 1.57 (↑) | 0.000 | 1.40 (↑) | 0.031 | 0.90 (↓) |
| (−) | [M − H]− | 834 | C18:0/C22:6 | 0.000 | 1.19 (↑) | 0.000 | 4.17 (↑) | 0.000 | 2.15 (↑) |
| (−) | [M − H]− | 836 | C18:1/C22:4 | 0.000 | 2.45 (↑) | 0.000 | 2.41 (↑) | 0.716 | 0.98 (ns) |
| (−) | [M − H]− | 838 | C18:0/C22:4 | 0.331 | 1.07 (ns) | 0.000 | 3.76 (↑) | 0.000 | 3.52 (↑) |
| (−) | [M − H]− | 842 | C18:1/C22:1 | 0.000 | 0.64 (↓) | 0.180 | 1.07 (ns) | 0.000 | 1.67 (↑) |
| (−) | [M − H]− | 844 | C18:1/C22:0 | 0.000 | 1.58 (↑) | 0.000 | 1.37 (↑) | 0.111 | 0.87 (ns) |
| PI | |||||||||
| (−) | [M − H]− | 807 | C16:0/C16:1 | 0.000 | 3.23 (↑) | 0.000 | 17.01 (↑) | 0.000 | 5.27 (↑) |
| (−) | [M − H]− | 833 | C16:1/C18:1 | 0.021 | 1.14 (↑) | 0.000 | 2.10 (↑) | 0.000 | 1.84 (↑) |
| (−) | [M − H]− | 835 | C16:0/C18:1 | 0.000 | 2.46 (↑) | 0.009 | 1.40 (↑) | 0.000 | 0.57 (↓) |
| (−) | [M − H]− | 861 | C18:1/C18:1 | 0.000 | 0.66 (↓) | 0.000 | 0.25 (↓) | 0.000 | 0.37 (↓) |
| (−) | [M − H]− | 863 | C18:0/C18:1 | 0.000 | 1.67 (↑) | 0.000 | 0.16 (↓) | 0.000 | 0.10 (↓) |
| (−) | [M − H]− | 881 | C16:0/C22:6 | 0.056 | 1.58 (ns) | 0.005 | 3.83 (↑) | 0.011 | 2.43 (↑) |
| (−) | [M − H]− | 883 | C18:1/C20:4 | 0.024 | 1.25 (↑) | 0.000 | 4.23 (↑) | 0.000 | 3.38 (↑) |
| (−) | [M − H]− | 885 | C18:0/C20:4 | 0.000 | 0.69 (↓) | 0.000 | 5.80 (↑) | 0.000 | 8.38 (↑) |
| (−) | [M − H]− | 887 | C18:0/C20:3 | 0.000 | 0.48 (↓) | 0.038 | 1.17 (↑) | 0.000 | 2.42 (↑) |
| PG | |||||||||
| (−) | [M − H]− | 747 | C16:0/C18:1 | 0.000 | 1.64 (↑) | 0.026 | 0.72 (↓) | 0.000 | 0.44 (↓) |
| (−) | [M − H]− | 773 | C18:1/C18:1 | 0.000 | 2.02 (↑) | 0.000 | 0.22 (↓) | 0.000 | 0.11 (↓) |
PC, phosphatidylcholine; plasmenyl-PC, plasmenylphosphatidylcholine; plasmenyl-PE, plasmenylphosphatidylethanolamine; PE, phosphatidylethanolamine; PS, phosphatidylserine; PI, phosphatidylinositol; PG, phosphatidylglycerol.
ns, no significant difference (p > 0.05); ↑, significant difference (p < 0.05) and relative fold change > 1; ↓, significant difference (p < 0.05) and relative fold change < 1.