Skip to main content
. 2016 Aug 24;7(41):67129–67141. doi: 10.18632/oncotarget.11562

Figure 4. ROS and JNK take part in H2-18-induced programmed cell death.

Figure 4

A. Immunoblots examining the phosphorylation of JNK and c-Jun in HCC-1954 and BT-474 cells treated with control IgG, trastuzumab, pertuzumab, pertuzumab plus trastuzumab, and H2-18. B. DCFH-DA or DHE was used to determine the ROS production in HCC-1954 cells treated with control IgG, trastuzumab, pertuzumab, pertuzumab plus trastuzumab, and H2-18. C. The effect of JNK inhibitor SP600125 or ROS scavenger NAC on H2-18-induced cell death in HCC-1954 cells. Data are shown as means ± SD. *, P<0.05; **, P<0.01; ***, P<0.001; ANOVA. D. JC-1 staining was used to measure the mitochondrial membrane potential in HCC-1954 cells treated with control IgG, trastuzumab, pertuzumab, pertuzumab plus trastuzumab, and H2-18. HCC-1954 cells treated with CCCP was used as positive control. Every experiment was repeated 3 times.