Figure 2. H22(scFv)-MAP induces apoptosis in CD64⁺ leukemic blasts ex vivo.

Leukemic blasts isolated from four AML patients A. and three CMML patients B. were treated ex vivo with 200 nM H22(scFv)-ETA’, H22(scFv)-MAP or Mock-MAP for 12 h. The leukemic cells were stained with Annexin V–eGFP and PI. Dot blots (ungated) show the specific pro-apoptotic effect of the H22(scFv)-MAP compared to Mock-MAP and H22(scFv)-ETA’. The experiment was carried out in duplicate.