Figure 4. Apoptosis and necrosis induction determined by flow cytometry of annexin V/PI double-stained glioblastoma and glioblastoma stem-like cells.

Treatment occurred with 50 μM TMZ and 15 μg/ml ART; for G112SP stem-like cells 2 μM TMZ was used. Cells were analyzed 144 h after the onset of TMZ treatment. ART was given to the medium 72 h after TMZ treatment of (A) LN229, (B) A172, (C) U87MG and (D) G112SP cells. All data are the mean +/− SD of at least three independent experiments.