Skip to main content
. 2016 Sep 15;7(41):67373–67386. doi: 10.18632/oncotarget.12033

Figure 1. Adenosine metabolism in glioblastoma stem-like cells.

Figure 1

Extracellular adenosine accumulation and AMPase activity in U87MG and PC of adherent glioblastoma cells (Adh) and their respective GSCs, measured by HPLC. (A) Measurements of extracellular adenosine accumulation in U87MG and PC Adh cells and GSCs at day seven of culture. (B) AMPase activity in U87MG and PC of Adh cells and GSCs exposed to AMP (400 nM) for 15 min in Tyrode's buffer at 37°C and controlled oxygen levels. Adenosine concentrations in A) and B) were normalized to the total protein concentration in each test. (C) Flow cytometry graph of A3AR on U87MG Adh cells and GSCs at day seven of culture (upper panel). Representative flow cytometry histogram is present (lower panel) (D) Western blot of A3AR on U87MG and PC Adh cells and GSCs at day seven of culture. Graphs represent the mean ± S.D. *P < 0.05 Adh versus GSCs; #P < 0.05 U87MG versus PC. n = 6.