Figure 5. Evaluation of adenosine signalling on cell viability and proliferation of glioblastoma stem-like cells.
Cell viability and proliferation were evaluated by MTT and 3H-thymidine incorporation assays respectively. (A–B) Cell viability in U87MG (A) and PC (B) Adh cells and their respective GSCs treated with AOPCP (50 mM), MRS1220 (10 μM), LY294002 (50 nM), PD98059 (50 μM) and Vc (100 nM) for 24 hrs. (C) Proliferation of U87MG Adh cells and their respective GSCs treated with AOPCP (50 mM), MRS1220 (10 μM) and Vc (100 nM) for 24 hrs. Cells treated with DMEM-0.001% DMSO (Vehicle) were used as the basal (“B”) condition. *P < 0.05 versus control. #P < 0.05 Adh versus GSCs. n = 5.