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. 2016 Sep 1;7(41):67387–67402. doi: 10.18632/oncotarget.11794

Figure 4. Metastatic colorectal cancer-derived exosomes sufficiently mediate cytoskeleton rearrangement of macrophages.

Figure 4

A. Morphological observation of F-actin rearrangement. Mouse bone marrow cells were differentiated for 3 d, followed by treatment with CT-26 CM or CT-26 exosomes (CT-26 Exo) for additional 3 d. As an interference, CT26 exosomes were disrupted in the freeze-thaw group by three quick free-thaw cycles. Images shown are F-actin (Red) and nuclei (Blue) staining by confocal microscopy. Scale bar = 20 μm. B, C. Statistical comparison of shape indices (B) and percentages of polarized cells (C). Image n = 20. D. Evaluation of EMT phenotypes of different cell types. E. Immunoblotting on the exosomal vimentin. Exosomes of the 4 cell lines were obtained by ExoQuick. Equal amounts of total exosome proteins (10 μg per cell line) were analyzed by IB on vimentin and CD9, respectively. F, G. Statistical analyses on shape indices (F) and percentage of polarized cells (G) exposed to exosomes acquired from different cell lines. Image n = 20. All statistical results shown in this Figure were obtained from 3 independent experiments. Data are shown as mean ± s.e.m. Statistical difference was tested by one-way ANOVA with Bonferroni post hoc multiple comparisons (two-tailed).