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. 2016 Sep 8;7(41):67449–67462. doi: 10.18632/oncotarget.11894

Figure 3. The effect of hyperthermia (45°C) and Hspb1 knock down on apoptosis and necrosis in B16 cell line analysed by flow cytometry.

Figure 3

A. Flow cytometric images of apoptosis and necrosis in B16 cells treated with hyperthermia and siHspb1-1 transfection. Cells stained with Annexin V+/ PI−, Annexin V+/ PI+, and Annexin-V−/PI+ were interpreted as early apoptotic cells, late apoptotic cells, and necrotic cells, respectively. B. Apoptosis and necrosis level of B16 cells were shown as fold change against siRNA negative group (37°C), and data shown were means from three independent experiments ± SD. * indicates a significant difference between each group and siRNA negative (37°C) (* p<0.05, by one way ANOVA); ^ siHspb1-1 (45°C) vs siHspb1-1 (37°C) (p<0.05, by student's t-test); # siHspb1-1 (45°C) vs siRNA negative (45°C) (p<0.05, by student's t-test).