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. 2016 Sep 24;7(45):72699–72715. doi: 10.18632/oncotarget.12236

Figure 1. Subcellular localization of cytoplasmic actins and microtubules in spreading epithelial cells.

Figure 1

HaCaT A.-D. or MCF-7 (E) cells were plated for either 6 (A, B, C) or 16 hours (D, E) and stained for β-actin, γ-actin and α-tubulin. Images represent single X/Y sections (A, C, D) and Z section (D, bottom image). Panel B and E represent galleries of optical sections taken with 0.5 μm (B) or 0.3 μm E. step from the ventral (close to the substrate, first image) to the dorsal (last image) side of the HaCaT (B) cell shown in Fig 1A or MCF7 cell (E). Microtubules are distributed in close proximity to the γ-actin network, but not codistributed with the β-actin bundles. Bars, 5 μm (C) and 10 μm.