Figure 3. Chloropyramine increases SASH1 expression in breast cancer cell lines.

(A–H) Cells were treated with 25 or 50 μM chloropyramine for 24 h, then lysates were prepared and SASH1 protein expression was analysed using immunoblotting. Immunoblot band intensities were quantified relative to β-actin in three independent experiments. The reproducibility and significance of changes in SASH1 expression with treatment were assessed using two-tailed t-tests. *p < 0.05, **p < 0.005.