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. 2016 Sep 14;7(45):72807–72818. doi: 10.18632/oncotarget.12020

Figure 4. Chloropyramine induces dose-dependent reduction of breast cancer cell line growth that involves apoptosis.

Figure 4

(A–H) Changes in adherent breast cancer cell line confluence following chloropyramine treatment. Cells were treated with chloropyramine for 96 h and imaged using light microscopy IncuCyte ZOOM system and digitally analysed to assess confluence relative to an untreated control culture. (I–K) Chloropyramine induces apoptosis in breast cancer cell lines. Cells were stained with propidium iodide and an Annexin V-FITC antibody conjugate 48 h post-chloropyramine treatment, and analysed by flow cytometry. All data shown are means +/− the standard deviation from three independent experiments. Statistical analysis was performed using two-tailed t-tests; *p < 0.05, **p < 0.005, ***p < 0.0005.