Table 3. Number of cells identified as big monocytes/macrophages in both control and Grp94-treated PBMCs of cancer patients.

patient	sex	average number of big cells/field
		control	Grp94
# 2	M		5	17
# 3	F		4	10
# 4	F		13	21
# 7	F		4	10
# 8	M		1	8
# 9	F		6	19
# 10	M		4	10
# 11	M		4	16
# 12	M		0	8
# 14	F		3	11
# 16	M		5	7
# 17	M		1	11
# 20	F		5	15
# 23	M		3	9
# 24	F		9	14
# 25	M		14	17
# 27	F		10	26
sex ratio	9M/8F	median	4	11
		(range)	(0–14)	(7–26)

The cell count was measured in listed patients whereas in missing ten patients a pattern of big agglomerates was the common feature in Grp94-treated (100 ng/ml) PBMCs that completely obscured the visualization of individual cells. Numbers represent the average of cells counted in at least four fields of both control and Grp94-treated PBMC images taken at the optical microscope at least in duplicate for each sample, and analyzed using ImageJ 1.51a (NIH, USA). The median (range) of control cells in males and females was, respectively, 4 (0–14) and 5.5 (3–13), whereas following Grp94 treatment was 5.5 (3–13) and 14.5 (10–26) in males and females, respectively. No difference was found between males and females for the cell number in both control (Mann-Whitney test, p = 0.12) and Grp94-treated PBMCs (p = 0.1), whereas a highly significant difference was present for the addition of Grp94 to PBMCs in both all patients (p = 5.77 × 10⁻⁵) and males (p = 0.003) and females (p = 0.004) considered separately.