Figure 6. Effects of Asp-UA on metastasis of breast cancer in vivo.

(A) a, Photography of the lung of animals inoculated with 4T1 breast carcinoma cells via tail vein; b, Hematoxylin–eosin staining assay represent the metastasis in the lungs, amplification ×50; c, Immunostaining with antibodies to CD44 was performed on lung sections from control mice, UA/Asp/Asp-UA treated mice, original magnification ×200. (B) Metastatic tumor nodule numbers in lung metastasis model. (C) Body weight change during treatment periods. (D) Quantitative of the mean CD44 positive area counted at ×200. The results were expressed as mean ± SD. (*P < 0.05, **P < 0.01 in comparison with control).