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. 2016 Sep 26;7(45):73147–73159. doi: 10.18632/oncotarget.12246

Figure 1. Adipocytes protect ALL from oxidative stress induced cell death.

Figure 1

(A) ALL cells co-cultured in TransWells over 3T3-L1 and ChubS7 pre-adipocytes (hatched bars) and adipocytes (black bars) with 72 hour DNR treatment (8093 35 nM, BV173 100 nM, RS4;11 100 nM, and Nalm6 200 nM). No feeder condition is where ALL cells were cultured alone (n = 3–5). (B) One representative image of western blot of Caspase 3 (37 kDa) and cleaved caspase 3 (19 and 17 kDa) in BV173 cells treated with DNR for 24 hours in the presence or absence of adipocytes. Images were histogram stretched in a consistent manner to increase brightness for publication. Quantification of the ratio of cleaved (both bands) over total caspase 3 (band at 37 kDa) is shown on the right (n = 3). (C) BV173 ALL cells cultured over 3T3-L1 cells and treated with various doses of doxorubicin (Dox). (D) 8093 cells treated with 35 nM DNR in 3T3-L1 ACM and ALCM (left, n = 6); BV173 cells treated with 100 nM DNR in ChubS7 ACM and ALCM (right, n = 5). (E) Quantification of cleaved over total caspase 3 of 8093 ALL cells after 24 hours treatment with 25 nM DNR with and without ALCM. (F) Annexin V staining of human ALL cells after 24 hour exposure to DNR with or without ALCM. *P < 0.05, **P < 0.01, ***P < 0.001 All asterisks indicate comparison to control no feeder (NF gray bar) or no DNR no adipocyte conditions (white bar) unless otherwise indicated.