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. 2016 Sep 26;7(45):73147–73159. doi: 10.18632/oncotarget.12246

Figure 2. Adipocytes protect ALL cells from oxidative stress.

Figure 2

(A) Measurement of % ROShigh population in Nalm6 cells treated with DNR for 6 hours. % ROShigh based on gates defined by cells without DNR treatment. Adipo condition is significantly different than both Fibro and No Feeder by repeated measure ANOVA (p < 0.05). (B) CellRox® intensity by flow cytometry measures intracellular ROS after 24 hours exposure to DNR or DNR plus ALCM. (C) GCLC and GCLM gene expressions by qPCR in 8093 and BV173 cells treated with DNR, with or without adipocytes present (n = 3). Asterisks and p-values indicate comparisons to the no-daunorubicin, no 3T3-L1 condition (white bar), unless otherwise noted. (D) ALL cells co-cultured with 3T3-L1 pre-adipocytes and adipocytes with chaetocin treatment (BV173 and Nalm6 100 nM, RS4;11 50 nM, n = 3–4). (E) Irradiated BV173 ALL cells co-cultured with 3T3-L1 pre-adipocytes and adipocytes (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.