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(A) Representative positive or negative sphere formation. (B–C) Percentage of the wells that form tumor sphere in a limited dilution assay of PANC-1 cells (B) or KPC-960 cells (C). (D) Western blot for Akt phosphorylation. (E) Cell viability in a CCK-8 assay at presence of gemcitabine. LY: LY29400. pAkt: phosphorylated Akt. *p < 0.05. N = 5.
