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. 2016 Sep 28;7(45):73229–73241. doi: 10.18632/oncotarget.12302

Figure 3. Palmitate activates Nlrp3 inflammasomes in MVECs.

Figure 3

(A) Representative Western blot documents and summarized data show the effects of palmitate (PA: 0–50 μM; 24 hours) on pro-caspase-1 (Pro-casp1) and cleaved caspase-1 (Cle-casp1) expression in MVECs (n = 4). (B) Summary of data for IL-1β production compared with untreated control (n = 4–6). *P < 0.05 versus control group. (C and D) Representative Western blot documents and summarized data show the effects of scramble or Nlrp3 gene silencing on caspase-1 processing (n = 3) and IL-1β production (n = 3–6) in MVECs stimulated with palmitate (20 μM; 24 hours). *P < 0.05 versus control group. *P < 0.05 vs. Scramble Ctrl; #P < 0.05 vs. Scramble with PA. (E) Summarized data the effects of palmitate (20 μM; 24 hours) or lipopolysaccharides (LPS, 1 μg/ml) on the relative mRNA levels of Nlrp3, ASC, and caspase-1 genes in MVECs. *P < 0.05 vs. Ctrl.