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. 2016 Sep 28;7(45):73347–73369. doi: 10.18632/oncotarget.12332

Table 1. Bisulfite pyrosequencing analysis.

Gene Primer sequence (5′→3′) Chromosome GenBank accession number Amplicon length [bp] No. and position of CpGs analyzed Primer concentration [nM] Ta [°C]
ABCB1 F: GTTGGAGGT GAGATTAATTTT 7 NG_011513.1 162 7 [117888–117952] 200 58.3
R: [Btn]AAACCCC CAACTCTACCT
S: GAGAGTAGTAA GAGGGA
ABCC1 F: TTTATAGGATGA AATGAGGGTATAGT 16 NG_028268.1 284 8 [4786–4831] 400 59.8
R: [Btn]AACAACCCA ACCAACCACCTCT
S: GTGTGTGGTTTT AAAGATT
ABCG2 F: GTTTGATTTAGTT GGGTTTGG 4 NG_032067.2 122 8 [77051–77093] 400 54.8
R: [Btn]AACCACCC ATTTAACTTACTCT
S: ATTTAGTTGGGTT TGGT