Table 1. Bisulfite pyrosequencing analysis.
Gene | Primer sequence (5′→3′) | Chromosome | GenBank accession number | Amplicon length [bp] | No. and position of CpGs analyzed | Primer concentration [nM] | Ta [°C] |
---|---|---|---|---|---|---|---|
ABCB1 | F: GTTGGAGGT GAGATTAATTTT | 7 | NG_011513.1 | 162 | 7 [117888–117952] | 200 | 58.3 |
R: [Btn]AAACCCC CAACTCTACCT | |||||||
S: GAGAGTAGTAA GAGGGA | |||||||
ABCC1 | F: TTTATAGGATGA AATGAGGGTATAGT | 16 | NG_028268.1 | 284 | 8 [4786–4831] | 400 | 59.8 |
R: [Btn]AACAACCCA ACCAACCACCTCT | |||||||
S: GTGTGTGGTTTT AAAGATT | |||||||
ABCG2 | F: GTTTGATTTAGTT GGGTTTGG | 4 | NG_032067.2 | 122 | 8 [77051–77093] | 400 | 54.8 |
R: [Btn]AACCACCC ATTTAACTTACTCT | |||||||
S: ATTTAGTTGGGTT TGGT |