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. 2016 Oct 4;7(45):73593–73606. doi: 10.18632/oncotarget.12435

Figure 3. Knockdown of NRF2 inhibits cell proliferation of breast cancer cells.

Figure 3

Cells were treated with siCtrl, siNrf2 or siCtrl together with Compound 1. (A–D) Cell proliferation was measured by Ki67 immunostaining. (A, B) Cells were stained with anti-Ki67 antibodies to detect cell proliferation ability (green), and with DAPI, to detect nuclei (blue). n = 5. (C, D) Ki67 staining rate was quantified by Image J. (E, F) Cell growth was measured using thiazolyl blue assay at various time points. n = 10, bar: SD, *P < 0.05; **P < 0.01; ***P < 0.005.