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. 2016 Oct 4;7(45):73593–73606. doi: 10.18632/oncotarget.12435

Figure 7. RhoA reverses the effect of NRF2 downregulation on cell metastasis.

Figure 7

Cells were treated with siCtrl, siNrf2, siNrf2 together with RhoA expressing vectors or siRhoA together with Compound 1. (A, C) Cell invasion capacity was evaluated by matrigel-coated transwell assays, scale bar: 50 μm. (B) Cell migration capacity was evaluated by transwell assay. (D, E) Scratch wound assays of control- (siCtrl) or other treatment MDA-MB-231 cells (D) or MCF7 cells (E). Phase contrast microphotographs are shown. Wound areas are shown as black bars, scale bar: 100 μm. (F, G) Quantification is displayed as percentage of maximal migration (area of wound closure in siCtrl-treated cells after 24 h). All data are means of three experiments, bar: SD, *P < 0.05; **P < 0.01; ***P < 0.005.