Table 2. Primers used in this study.
| Application | Primer name | Primer sequence | Tm (°C) |
|---|---|---|---|
| 3′RACE | GHR-AS3(GSP1) | GGGTCAATCCCTTTAATCTTT | 52 |
| GHR-AS3(NGSP1) | CAACAACTAAGAACCAGGGAAA | ||
| 5′RACE | GHR-AS5(GSP1) | TCCTCCTGTGCCAGTTCC | 52 |
| GHR-AS5(NGSP1) | GCGTGTTCAGGAGCAAAGCT | ||
| GHR-AS full length PCR | GHR-AS-F | TTTTTTTTTTTTTTTTTT | 52 |
| GHR-AS-R | GTATGAAGAGTCCCAACCAAC | ||
| GHR-AS quantitative PCR | qGHR-AS-F | TTGCTAATGTTTCTGTTCTGTG | 56.3 |
| qGHR-AS-R | GGGTCAATCCCTTTAATCTTT | ||
| GHR-S quantitative PCR | qGHR-S-F | AGTCCGATCAAGACAACGTAC' | 56.3 |
| qGHR-S-R | CTAAGAACCAGGGAAACTCG | ||
| ZNF131 quantitative PCR | ZNF131-F | ATGTCCAAACTGCCACG | 56.3 |
| ZNF131-R | CACGCTGTTACAAACCTGA | ||
| DNA contamination detection | β-actin-F | TCATTGTGCTAGGTGCCA | 50–60 |
| β-actin-R | CCTCTTCCAGCCATCTTT | ||
| Internal control in qPCR analysis | GAPDH-F | TCCTCCACCTTTGATGCG | 50–60 |
| GAPDH-R | GTGCCTGGCTCACTCCTT | ||
| Overlapping region(1) detection | OL1-F | TTTCCCTGGTTCTTAGTTGTTG | 55 |
| OL1-R | GGGTCAATCCCTTTAATCTTT | ||
| Overlapping region(2) detection | OL2-F | GCGTGTTCAGGAGCAAAGCT | 60 |
| OL2-R | TGGGACAGGCATTTCCATACTT | ||
| ChIP-qPCR | GHR-S promoter-V1-F | GCCTATAGCTGTCGCCTA | 60 |
| GHR-Spromoter-V1-R | GGAGAGCACTGTCTGATG | ||
| DNA methylation analysis | GHR- S promoter-BSP-F1 | GAGGGCGGTCGTTGTTCG | 60 |
| GHR- S promoter-BSP-R1 | TTCTCCGCAACGCCCGCTCGTC | ||
| GHR- S promoter-BSP-F2 | GTGATATTTAAGTAAAATAAATTGT GGGAT | 66–56 (−0.5°C /cycle) | |
| GHR- S promoter-BSP-R2 | ACTCRACRCATTCCTAAAAACRCR ACTAACC |