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. 2016 Sep 15;7(45):73664–73680. doi: 10.18632/oncotarget.12046

Figure 2. RNAi knockdown of TP53 and PARP1 shows synergistic killing in both CL1-5 and H1975 cells.

Figure 2

A. 2000 of A549, CL1-5 and H1975 cells were infected with lentivirus containing the indicated shRNAs for 72 hours. Cell viability was measured by MTT assay and compared to the control shLacZ group, the number of replicates (n) was nine per group. Values are reported as the mean ± standard error, where ** and *** denote p-value < 0.01 and < 0.001 from two-sample t-test, respectively. B. RNAi knockdown of TP53 and PARP1 by the indicated shRNA lentivirus in A549, CL1-5 and H1975 cells for 72 hours. Cell lysates were analyzed by western blotting using the indicated antibodies. Cleaved PARP and active caspase 3 were the apoptotic indicators and β-actin was the internal control. C. The long-term effects of RNAi knockdown of TP53 and PARP1 were examined by colony formation assay. 250 of A549, CL1-5 and H1975 cells infected with lentivirus containing the indicated shRNAs were cultured for 10 days. Colonies were stained by crystal violet and counted. n = 3 per group, and *** denotes p-value < 0.001 from two-sample t-test.