Table 1. List of RT-PCR and siRNA oligonucleotides.
Target gene | Sequence |
---|---|
RT-PCR | |
Human β-actin | FWD-5′- CATGTACGTTGCTATCCAGGC REV-5′- CTCCTTAATGTCACGCACGAT |
Human p21 | FWD-5′- TGTCCGTCAGAACCCATGC REV-5′- AAAGTCGAAGTTCCATCGCTC |
Human Survivin | FWD-5′-GATGACGACCCCATAGAGGAAC REV-5′-GGGTTAATTCTTCAAACTGCTTCT |
Human CD44 | FWD-5′- CTGCCGCTTTGCAGGTGTA REV-5′- CATTGTGGGCAAGGTGCTATT |
Human CD133 | FWD-5′- AGTCGGAAACTGGCAGATAGC REV-5′- GGTAGTGTTGTACTGGGCCAAT |
Human CD90 | FWD-5′-GGATACCAGGAGTTATTGGAGAAG REV-5′-CTTGGCTCTCCTGGATGTATTT |
Human ANPEP | FWD-5′-GACCAGTACAGCGAGGTTAATG REV-5′-GGGTGGATCGGGTTATTATTGG |
Human GPC3 | FWD-5′-CCACCACTGAAACTGAGAAGAA REV-5′-GTTGTTGAGAATGGGCACATAAC |
Human AFP | FWD-5′-GGATACCAGGAGTTATTGGAGAAG REV-5′-CTTGGCTCTCCTGGATGTATTT |
Human ID1 | FWD-5′-TTACGTGCTCTGTGGGTCTC REV-5′-CCCCCTAAAGTCTCTGGTGA |
Human ID2 | FWD-5′-ATGAAAGCCTTCAGTCCCGT REV-5′-TTCCATCTTGCTCACCTTCTT |
Human ID3 | FWD-5′-TCATCTCCAACGACAAAAGG REV-5′-ACCAGGTTTAGTCTCCAGGAA |
Human ID4 | FWD-5′-CGCTAAGCTACTGTCCAATCTC REV-5′-ATGAGACCAGAAATCACAGTACAA |
siRNA | |
Human ID1 | FWD-5′-UCGCAUCUUGUGUCGCUGA(dTdT) REV-5′-UCAGCGACACAAGAUGCGA(dTdT) |
Human ID2 | FWD-5′-CUUACUUGGACUGUGAUAU(dTdT) REV-5′-AUAUCACAGUCCAAGUAAG(dTdT) |
Human ID3 | FWD-5′-CUGUAACAAUGCGAUGUAU(dTdT) REV-5′-AUACAUCGCAUUGUUACAG(dTdT) |
Human ID4 | FWD-5′-GUGACAUUUCAUACCAUGU(dTdT) REV-5′-ACAUGGUAUGAAAUGUCAC(dTdT) |